Deteksi metilasi DNA genom Elaeis guineensis Jacq hasil kultur jaringan dengan teknik Randomly Amplified Fingerprint (RAF) DNA dan Reverse Phase HPLC (RP-HPLC) Analysis DNA genom methylation of Elaeis guineensis Jacq from tissue culture by Randomly Amplified Fingerprint DNA (RAF) and Reverse Phase HPLC (RP-HPLC)

A validated RP-HPLC Method for Quantification of Alpha-tocopherol in Elaeis guineensis Leaf Extracts

Background: Elaeis guineensis (oil palm) is a tropical tree from the family Arecaceae. The tree is rich in vitamin E such as alpha-tocopherol, an essential antioxidant in the lipid phase of the human body. Objective: This study aims to develop a reverse phase HPLC method for determination of alpha-tocopherol in E. guineensis leaf extracts. Methods: This method was validated at 220 nm for linear...

Development of Randomly Amplified Polymorphic DNA Based SCAR Marker for Identification of Ipomoea mauritiana Jacq (Convolvulaceae)

Vidari is an Ayurvedic herbal drug used as aphrodisiac, galactagogue and is also used in the preparation of Chyavanaprash. Tubers of Ipomoea mauritiana Jacq. (Convolvulaceae), Pueraria tuberosa (Roxb. ex Willd.) DC (Fabaceae), Adenia hondala (Gaertn.) de Wilde (Passifloraceae) and pith of Cycas circinalis L. (Cycadaceae) are all traded in the name of Vidari, creating issues of botanical authent...

Study of Genetic Diversity among Philodendron Varieties by Randomly Amplified Polymorphic DNA Markers

In the present study, Randomly Amplified Polymorphic DNA (RAPD) markers were used to analyze the genetic diversity in 20 varieties of Philodendron. The polymerase chain reaction was performed with 60 RAPD primers, out of which 21 primers showed clear amplification as well as more polymorphism. In total, 354 scorable RAPD loci with 348 polymorphic bands (98%) were observed. Percentages of polymo...

DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA.

DNA profiles of 40 sugarcane genotypes were constructed with 30 RAPD markers. Sugarcane genotypes of both Saccharum officinarum and S. barberi were included in this study. Multiple alleles were detected from each RAPD; there was a high level of polymorphism. On average, 7.93 alleles were produced per primer, giving a total of 238 alleles. The genetic distances between these genotypes were asses...

Control Approaches against Vascular Wilt Disease of Elaeis guineensis Jacq. Caused by Fusarium oxysporum